Fig. 2: Phase behavior of LplA in E. coli cells. | Nature Communications

Fig. 2: Phase behavior of LplA in E. coli cells.

From: A globular protein exhibits rare phase behavior and forms chemically regulated orthogonal condensates in cells

Fig. 2

a Confocal images of mEGFP-labeled endogenous LplA in E. coli MG1655 under various cellular stress conditions. Scale bars, 5 μm. Images are representative of three biological replicates. b The overexpressed LplA in E. coli BL21(DE3) formed pole-distributed condensates, with 1,6-hexanediol being able to dissolve the condensates to certain extent. Scale bars, 5 μm. c Quantification of percent protein in condensates in experiment shown in (b). Data are expressed as the mean ±s.d. for for n = 5 cells from three independent experiments. d Normalized FRAP analysis of LplA- mEGFP condensates in E. coli. Data are expressed as the mean ±s.d. for data (n = 6 cells) from three independent experiments. Scale bars, 5 μm. Source data are provided as a Source Data file.

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