Fig. 4: Responsiveness of LplA phase separation to lipoic acid and its analogues. | Nature Communications

Fig. 4: Responsiveness of LplA phase separation to lipoic acid and its analogues.

From: A globular protein exhibits rare phase behavior and forms chemically regulated orthogonal condensates in cells

Fig. 4

a Chemical structures of (R)-lipoic acid (LA, 1) and its analogues: (R/S)-lipoamide (LM, 2), octanoic acid (OA, 3), octanamide (OM, 4), 1,3-propanedithiol (PDT, 5). 1,6-hexanediol (HDO, 6) is used here as a positive control for non-specific destruction of protein condensates. b Phase behavior of purified LplA (500 μM LplA in Tris buffer) in the presence of several small molecules in vitro. Before the turbidity measurement, we performed a 5-min shaking of the samples to ensure homogeneity. Data are representative of three independent experiments. Source data are provided as a Source Data file. c Phase behavior of LplA in the presence of several small molecules in E. coli. Scale bars, 5 μm. Images are representative of three biological replicates.

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