Fig. 3: Functional validation of two master regulators.
From: The regulatory architecture of the primed pluripotent cell state
a Morphology of KO or control EpiSC lines; representative images for independent lines are shown. b Fold change in cell number after five days of colony formation. c Number of alkaline phosphatase positive colonies. d Time line of neuroectoderm differentiation. e Immunofluorescence staining of biological replicates (clonal lines) for lineage-specific markers. Each MR was characterized by two clonal KO cell lines in at least three independent replicates. f Time line of mesendoderm differentiation. g Staining for lineage-specific markers; number of replicates as in (e). h Western blots for Cbl and Zc3h13. Source data are provided as a Source Data file. i Schematic of the teratoma assay. j Representative images of mutant and control teratomas dissected from contralateral legs of the same animal. k Teratoma weight. l Hematoxylin and eosin (H&E) and immunohistochemical staining of serial sections. m, n Fraction of area showing ectoderm (neural rosette) (m) and endoderm (ciliated epithelium) features (n). Line and whiskers show the mean ± s.d.; statistical significance is shown as FDR, 2-tailed U-test. Scale bars: 100 microns.
