Fig. 3: CuII→I reduction kinetics of A10W mutants monitored using Trp emission. | Nature Communications

Fig. 3: CuII→I reduction kinetics of A10W mutants monitored using Trp emission.

From: Controlling outer-sphere solvent reorganization energy to turn on or off the function of artificial metalloenzymes

Fig. 3

Cartoon showing the A10W mutation in 3SCC (A) and 4SCC (B) to bring the Trp closer to Cu. C Reduction of CuII-A10W-3SCC (red) and CuII-A10W-4SCC (blue) by Asc. D The kobs depend linearly on [Asc] with a significantly higher rate in 3SCC than in 4SCC. Here, 0.125 mM CuII-ArCuPs were anaerobically mixed with varying [Asc] in 10 mM MES buffer pH 6.5, and time-dependent emission was monitored (C shows the data with 10-fold Asc). The error bars in D represent SD from three independent measurements. λex = 280 nm, λem = 340 nm for 3SCC and λem = 345 nm for 4SCC.

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