Fig. 2: LDHB suppresses mitochondria-associated ferroptosis in cancer cells. | Nature Communications

Fig. 2: LDHB suppresses mitochondria-associated ferroptosis in cancer cells.

From: Ubiquinol-mediated suppression of mitochondria-associated ferroptosis is a targetable function of lactate dehydrogenase B in cancer

Fig. 2

Heatmap of cell viability and images of the clonogenic assay of siRNAs cells treated with RSL3 alone or in combination with 5 µM FER1 for 48 h after pretreatment with vehicle or 5 µM FER1 for 24 h (a, b). Assessment of cell viability with PrestoBlue in A549, HT1080, PANC-1, MSTO-211H siRNAs cells treated with DMSO or RSL3 alone or in combination with 20 µM mitoTEMPO, 20 µM TEMPO, 5 µM FER1 for 48 h after pretreatment with vehicle, 20 µM mitoTEMPO, 20 µM TEMPO, 5 µM FER1 for 24 h, n = 3 independent replicates (c). Assessment of mitochondrial lipid peroxidation by flow cytometry in A549, HT1080, PANC-1, MSTO-211H siRNAs cells treated with DMSO or RSL3 alone (1 µM for A549, 0.75 µM for HT1080, and MSTO-211H cells, 0.5 µM PANC-1) or in combination with 20 µM mitoTEMPO, 20 µM TEMPO, 5 µM FER1 for 1 h after pretreatment with vehicle, 20 µM mitoTEMPO, 20 µM TEMPO, 5 µM FER1 for 24 h, n = 4 independent replicates for A549, PANC-1, MSTO-211H cell lines treated with DMSO and n = 3 independent replicates for the rest groups, n = 3 independent replicates for HT1080 cell line (d, e). Analysis of PI staining by flow cytometer in A549, HT1080, PANC-1, MSTO-211H cells after 72 h of transfection with siRNAs treated with DMSO or RSL3 alone (1 µM for A549, 500 nM for HT1080, 250 nM for MSTO-211H, 50 nM for PANC-1) or in combination with 5 µM FER1, 20 µM mitoTEMPO, 20 µM TEMPO for 24 h. n = 3 independent replicates (f). Data were presented as mean ± SD. Two-way ANOVA (c), Unpaired, two-tailed t-test (e, f). ns no significant difference, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Source data are provided as a Source Data file.

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