Fig. 3: Epigenomic controllers maintain durable repression in vivo.

A In C57BL/6 female mice, a single 3 mg/kg dose of Pcsk9-EC led to a reduction in Pcsk9 serum levels (orange) compared to control mice (black) as measured by ELISA for up to 180 days. Serum was sampled every 14 days post-treatment (n = 10 mice in each cohort, ****P < 0.0001, t test). B Pcsk9 promoter methylation was increased in liver punches from Pcsk9-EC treated mice (orange) compared to control mice (black) up to 180 days post-treatment (n = 10 mice in each cohort; ****P < 0.0001, t test). Source data are provided as a Source Data file.