Fig. 2: A HFD promotes vascular leakiness and fibronectin expression.
From: The impact of a high fat diet and platelet activation on pre-metastatic niche formation
A Representative images of P-Selectin staining in the lungs of ND and HFD-fed mice. Bottom panels are magnifications of the boxed area. Bar: 50 μm (B) Quantitative analysis of P-Selectin staining in the lungs of ND and HFD mice. 2 independent experiments: ND n = 9 fields, HFD n = 10 fields per group. C Representative images of the vascular permeability in the lungs of ND and HFD mice visualized with NIR-Dextran. D Quantification of (C). 3 independent experiments: n = 11 mice per group. E Heatmap of genes in endothelial cells from ND and HFD mice based on RNA-seq data: n = 3 mice per group. F Correlation between the RNA-seq data and the proteomic data from ND and HFD mice (FDR < 0.05) and fold change. G Representative images of FN1 staining in the lungs of ND and HFD mice. Bottom panels are magnifications of the boxed area. Bar: 100 μm. H Quantification of G. 2 independent experiments: n = 14 fields per group. I Representative Western blot of FN1 in whole lung extracts comparing non-perfused and perfused lungs from both ND and HFD mice. J Quantification of the experiments in I. Data correspond to 2 independent experiments ND (n = 8 perfused and n = 8 non-perfused) and HFD (n = 8 perfused and n = 7 non-perfused). Representative Western blot of FN1 in plasma (K) and platelets (L) isolated from ND and HFD mice. M, N Quantification of the experiments in K and L respectively. 2 independent experiments, M (ND n = 7, HFD n = 8), N (ND n = 7, HFD n = 6) platelet samples per group. O) Analysis of platelet fibronectin uptake by flow cytometry expressed as total counts (P) or MFI (Q). The data corresponds to 4 independent experiments: n = 3 platelet samples per group. All the data represent the mean ± s.d., and the P values were calculated with a two-tailed Student’s t-test (B, D, H, M, N, P, Q) or Mann-Whitney test (J). Values are shown in box‐and‐whisker plots where the line in the box corresponds to the mean. Boxes extend from the minimum to the maximum value.
