Fig. 1: Blocking capacity of the VHH constructs against the CTXB–GM1 interaction.
From: Orally delivered toxin–binding protein protects against diarrhoea in a murine cholera model
a The ability to block the CTXB–GM1 interaction for selected monomeric VHHs at a molar ratio of 10:1 (VHH:CTXB). The average blocking effect of each VHH was calculated from a single measurement of technical duplicates normalized against a control mixture containing CTXB and a VHH without specificity for CTXB. Error bars represent standard deviation. Source data are provided as a Source Data file. b The CTXB-blocking capacity of the monovalent BL3.1 in comparison to the bivalent (VHH–(G4S)3–VHH) BL3.2 at molar ratios of 1:1, 10:1, and 100:1 (VHH construct:CTXB). A previously reported anti-CTX VHH control and a negative VHH control without specificity for CTXB were included as well42. The average blocking capacity was calculated from duplicate measurements of technical triplicates. Error bars represent standard deviation. Source data are provided in a Source Data file.
