Fig. 2: Thermal and simulated gastrointestinal stability profile of BL3.2.
From: Orally delivered toxin–binding protein protects against diarrhoea in a murine cholera model
a CTXB-binding activity of BL3.2 after incubation for up to 4 h in simulated gastric fluid (SGF; pH 1.2) or up to 5 h in simulated intestinal fluid (SIF; pH 6.8) at 37 °C. Binding activity was normalized against a BL3.2 control stored at 4 °C in phosphate-buffered saline (PBS) for 5 h. The average CTXB-binding activity is based on measurements from two experiments of one sample per condition (SGF, SIF, and PBS) analysed in technical duplicates. Error bars represent standard deviation. Source data are provided in a Source Data file. b Thermal denaturation temperature (Tm) of BL3.2 and CTX in PBS. Each sample was analysed in triplicates, with lines indicating the averages.
