Fig. 3: Functional neutralization of CTX by BL3.2 in a human colon adenocarcinoma cell assay.
From: Orally delivered toxin–binding protein protects against diarrhoea in a murine cholera model
Reduction of intracellular cyclic adenosine monophosphate (cAMP) production in HCA-7 cells incubated with CTX (0.115 nM) pre-mixed with increasing concentrations (0.240–31.25 nM) of the bivalent VHH construct BL3.2 (circles) or a non-specific bivalent VHH construct control (triangles). Levels of intracellular cAMP were interpolated from a sigmoidal four parameter logistic cAMP standard curve (R2 = 0.9543) based on triplicate measurements. Each data point represents an interpolated mean value from biological duplicates comprised of three technical replicates. Source data are provided in a Source Data file.
