Fig. 5: Hydrogen–deuterium exchange mass spectrometry (HDX–MS) analysis of the CTX epitope of BL3.1.
From: Orally delivered toxin–binding protein protects against diarrhoea in a murine cholera model
a The level of HDX for CTXB alone and CTXB bound to BL3.1 with regards to two specific regions of the toxin: positions 29–38 and positions 50–56. The solid and dashed lines show the deuterium incorporation for CTXB and the CTX–BL3.1 complex, respectively. Each time point (1, 10, 40 and 100 min) was analysed in technical triplicates. Data are presented as mean values with standard deviation. b Surface representation of the CTXB pentamer. Shades of grey represent no HDX differences upon binding BL3.1. The level of HDX protection upon binding BL3.1 is indicated with different shades of blue. c A ribbon representation of a single subunit of CTXB with mapped HDX protection in shades of blue. A galactose molecule is shown in yellow, indicating the site of interaction with the intestinal cell receptor GM1.
