Fig. 1: The presence of the complement system significantly increased SARS-CoV-2 neutralisation titres in the OPTIC vaccinee cohort. | Nature Communications

Fig. 1: The presence of the complement system significantly increased SARS-CoV-2 neutralisation titres in the OPTIC vaccinee cohort.

From: Complement-mediated enhancement of SARS-CoV-2 antibody neutralisation potency in vaccinated individuals

Fig. 1

50% neutralisation titres (NT50) were determined via microneutralisation assays for all OPTIC vaccinee serum samples following the addition of media-only (DMEM/MEM), heat-inactivated (HI)-FCS, or pooled human plasma (PHP). a Vero E6 cells infected with VIC01 (n = 10) (PHP vs. DMEM, p = 0.0002; PHP vs. HI-FCS, p = 0.0002); b Vero E6 cells infected with BA.1 (n = 8) (PHP vs. DMEM, p = 0.0070; PHP vs. HI-FCS, p = 0.0063); c Calu-3 cells infected with VIC01 (n = 10) (PHP vs. MEM, p = 0.0050; PHP vs. HI-FCS, p = 0.0043); d Calu-3 cells infected with BA.1 (n = 9) (PHP vs. DMEM, p = <0.0001; PHP vs. HI-FCS, p = <0.0001). Each spot shows the average NT50 value for each sample determined by a 4-parameter logistic curve from four replicates across duplicate assays. Error bars show the mean with the standard error. Significance was determined using a one-way ANOVA with Geisser-Greenhouse correction and Tukey’s multiple comparisons test. e Log2 fold-changes in NT50 between HI-FCS and PHP represents the enhancement of neutralisation via the complement system for each condition shown in (a–d) (Vero E6/BA.1 vs. Calu-3/BA.1, p = 0.0015; Calu-3/VIC01 Vs Calu-3/BA.1, p = 0.0210; Calu-3/VIC01 Vs. Vero E6/VIC01, p = <0.0001). Statistical significance was determined using a one-way ANOVA mixed effects analysis with Geisser-Greenhouse correction and Šídák’s multiple comparisons test. Error bars show the mean value with standard deviation. For (a–e), arbitrary values of 10 were used for samples with a predicted NT50 below this value. If an NT50 value could not be determined in any condition, then the sample was omitted. f Significant differences in NT50 were determined for each individual using the sum-of-squares F-test with non-overlapping 95% confidence intervals. The outside number shows the total sample size and the centre number (shown as a percentage in red) reports the number of individuals with a significant increase in NT50 following the addition of PHP. All results were analysed and presented using GraphPad Prism (Version 10) and Inkscape. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.

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