Fig. 2: Distance Fluctuation (DF) and Shortest Path Map (SPM) analysis on M.
From: Pathogenic mutation impairs functional dynamics of Hsp60 in mono- and oligomeric states
a Shortest Path Map (SPM) of MWT and MV72I. Here SPM spheres are coloured according to the ΔDF Pavg value of the corresponding residues (see main text). The colour code is illustrated in the colour bar. b Percentage DF difference matrix ∆DF obtained from DFV72I–DFWT and taking DFWT as reference for percentage calculations. Blue areas (positive values) correspond to loss of allosteric coordination in V72I whereas orange ones (negative values) indicate a gain in coordination in V72I. White areas are those unaffected by the mutation. Residue numbering is shown on the axis together with the corresponding domain: Equatorial (residues 0–133,410–525); Intermediate (residues 134–188,374–409); Apical (residues 189–373). To integrate this ∆DF matrix, as Supplementary Movie 6, we provide a video of each of its columns (one column = one residue) projected onto the starting structure of M. c (Pavg) Per-residue average change in DF score upon V72I mutation, projected onto the starting structure of M. The colour code is illustrated in the colour bar. d (P72) Per-residue change in DF score with respect to residue 72, upon V72I mutation, projected onto the starting structure of M. The colour code is illustrated in the colour bar.
