Fig. 1: Cryo-EM structure determination of ETB using a three-point fusion strategy. | Nature Communications

Fig. 1: Cryo-EM structure determination of ETB using a three-point fusion strategy.

From: Structure of a lasso peptide bound ETB receptor provides insights into the mechanism of GPCR inverse agonism

Fig. 1

a Concept design of the three-point fusion strategy. b Purification of the ETB-CN-FKBP12 complex. Protein purifications were successfully reproduced at least twice. c, d Representative 2D cryo-EM averages of the ETB–CN-FKBP12 complex in the apo state (c) and bound to RES-701-3 (d). e, f Cryo-EM density maps and 3D models of the ETB–CN-FKBP12 complexes in the apo state (e) and complex with RES-701-3 (f), viewed from the side and top.

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