Fig. 3: Cholesterol-induced lipid packing modulates condensate wetting.

a Spectral phasor plot for GUVs made of DOPC and 0%, 5%, 15%, and 30% mol of cholesterol and containing 0.5 mol% LAURDAN at (23 ± 1)°C. b Pixel distribution histogram along the linear trajectory drawn as a white dotted line in (a), showing the fluidity fraction for the different membrane compositions. Data are represented as the mean (dots and lines) ± SD (shadowed contour), n  =  5 independent experiments per condition. c Center of mass of the histograms shown in (b). Individual data points are shown for each membrane composition. The lines indicate the mean value ± SD (n  =  5). The statistical analysis was performed with One-way ANOVA and Tukey post-test analysis (p  <  0.0001, **** | p  <  0.001, *** | p  <  0.01, ** | p  <  0.05, * | ns = non-significant). d Upper panel: cursor colored images of GUVs of the indicated compositions, corresponding to the cursors shown in (a). Lower panel: representative confocal microscopy images of GUVs labeled with 0.1 mol% ATTO 647N-DOPE (green) of the indicated compositions in contact with condensates labeled with FITC-glycinin (magenta) at the working conditions. Scale bars: 5 µm. e Geometric factor and affinity contrast for condensate/membrane systems at the different membrane compositions. Individual data points are shown for each membrane composition. The lines indicate the mean value ± SD (n  =  5). f Intrinsic contact angle for condensate/membrane systems at the different membrane compositions. Individual data points are shown for each membrane composition. The lines indicate the mean value ± SD (n  =  5). Source data are provided as a Source Data file.