Fig. 4: Lipid packing determines wetting phase specificity in phase-separated membranes.
a Sketch illustrating that in the presence of gel/fluid (Ld) or liquid-ordered (Lo)/liquid-disordered (Ld) phase-separated GUVs, the condensates bind exclusively to the liquid-disordered (Ld) phase. b–d Confocal sections (x,y), line profiles, and 3D projections (x,y,z), of phase-separated GUVs showing that glycinin condensates (magenta) only interact with the liquid-disordered phase (green), excluding the gel or the liquid-ordered phase. Scale bars: 5 µm. GUVs were labeled with 0.1 mol% ATTO647 N-DOPE and the lipid compositions are: (b) DOPC:DPPC 1:1; (c) DPPC:DOPC:Chol 1:1:1 and (d) DPPC:SM:Chol 1:1:1; cross-sections and 3D projections correspond to the same vesicle-condensate couple with the specific membrane composition. The line profiles show that condensates are always interacting with the membrane segments of highest intensity which corresponds to the Ld phase. Dashed lines in the 3D projections are a guide to the eye indicating the vesicle contour. See also Supplementary Movies 1–3. All images were taken under the working conditions defined above. Source data are provided as a Source Data file.
