Fig. 3: INa, IK1 and IKr recordings in iPSC-CMs. | Nature Communications

Fig. 3: INa, IK1 and IKr recordings in iPSC-CMs.

From: Comprehensive promotion of iPSC-CM maturation by integrating metabolic medium with nanopatterning and electrostimulation

Fig. 3

a Representative INa traces recorded under 25 mM extracellular Na+ concentration. b Statistical analysis of INa in single cells derived from 9 (B27) and 5 (MM, MM + NP, MM + NP + ES) independent differentiations of 3 iPSC lines. The stimulation protocol is shown as an inset. The holding potential was set at −100 mV. INa was recorded by increasing the test potential from −80 mV to +70 mV in 5 mV steps. Each pulse lasted for 20 ms and the sweep interval was 2 s. c Representative traces of 0.5 mM BaCl2-sensitive IK1 for different groups. d Statistical analysis of BaCl2-sensitive IK1 in single cells derived from 6 (B27, MM, MM + NP + ES) and 5 (MM + NP) independent differentiations of three iPSC lines. The stimulation protocol is shown as an inset. The holding potential was set at −40 mV. IK1 was recorded by increasing the test potential from −130 mV to +10 mV in 10 mV steps, with each pulse lasting for 2 s. The sweep interval was 10 s. The protocol was then repeated in the presence of 0.5 mM BaCl2 and the Ba2+-sensitive current was calculated as IK1. e Shown are traces of 1 µM E−4031-sensitive IKr in the four groups. f, g Averaged E−4031-sensitive IKr step currents (f) and tail currents (g) in single cells derived from 4 independent differentiations of 2 iPSC lines. The IKr pulse stimulation is shown as an inset. The holding potential was set at −50 mV. IKr step currents were recorded by decreasing the test potential from +40 mV to −40 mV in 10 mV steps with each pulse lasting for 2.5 s. The tail currents were recorded in the following 4-s phase at −40 mV. The sweep interval was 10 s. With respect to INa, IK1, and IKr, we did not observe significant differences among three iPSC lines used (Supplementary Fig. 7). Source data are provided as a Source Data file. Two-way ANOVA with Sidak’s multiple comparison test was used for statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to the B27 group. Exact p values are provided in the Source Data file. Data are presented as mean ± SEM.

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