Fig. 5: Starch production and composition profiles in micro-grain produced through low-carbon microbial manufacturing.

a and b Total starch production and starch composition in engineered cell cultured in MMA through shake flask cultivation. c and d Starch production and starch composition of ST1271 strain cultivated in MMA via fed-batch fermentation. e Schematic illustration of starch production from low-carbon corn stover feedstock through an engineered yeast-based conversion. [Created in BioRender. Shi, Z. (2025) https://BioRender.com/g62b742]. f Starch production and carbon source-to-starch yield of ST1271 strain cultivated in A, H, and H + A media for 132 h. ST1271 was cultivated in MM containing various 6.7 g/L acetate (A), corn stover hydrolysate (H, containing 12.60 g/L glucose and 0.25 g/L acetate), or corn stover hydrolysate and 6.7 g/L acetate (H + A). Data shown are mean values ± SDs of biological replicates (a (n = 4), c and d (n = 2), f (n = 3)), or mean values of biological replicates (b, n = 3 or 4). Carbon to starch yield data are presented as median values with interquartile range (IQR) and distribution of biological replicates (f, n = 3). Source data are provided as a Source Data file.