Fig. 6: Metabolomic and transcriptomic analysis to elucidate the cellular response ensuring high-level starch production.

a Comparison of metabolites and gene expression for central biosynthetic pathways for acetate-to-starch conversion in engineered strains ST587 and ST1271. b Transcription factors with the greatest expression changes. c GO enrichment of differentially expressed genes relating to amino acid metabolism and protein synthesis. d–f Cellular macromolecule comparison of the engineered strains. g Brief illustration of key nodes in the lipid biosynthetic pathway. h Starch content of strains engineered for decreased ACC1 expression. i Starch content of ST1220 strain treated with cerulenin. Starch content comparisons were conducted at different time points due to the growth inhibition caused by the addition of cerulenin (Supplementary Fig. 32). Data shown are a comparison of omics data of quadruplicates (a–c) or mean values ± SDs of biological replicates (d–f (n = 4), h (n = 3 or 4), i (n = 2 or 4)). Statistical difference was determined by a two-tailed unpaired t-test. PYR pyruvate, OAA oxaloacetate, 2-OG 2-oxoglutarate, PEP phosphoenolpyruvate, Glycerate-2P glycerate 2-phosphate, Glycerate-3P glycerate 3-phosphate, Glycerate-1,3P2 glycerate 1,3-diphosphate, G3P glyceraldehyde 3-phosphate, DHAP dihydroxyacetone phosphate, 6PG gluconate 6-phosphate, Ru5P ribulose 5-phosphate, Xu5P xylulose 5-phosphate, R5P ribose 5-phosphate, S7P sedoheptulose 7-phosphate, E4P erythrose 4-phosphate, G1P glucose 1-phosphate, ADP-glu ADP-glucose, UDP-glu UDP-glucose. Source data are provided as a Source Data file.