Fig. 7: Distal effects of wf-UMP induced synergistic immunotherapy and T cell-mediated anti-tumor immunity.

a Schematic illustration of bilateral tumor treatment strategies. Created in BioRender. Jin, J. (2025) http://BioRender.com/x39n446. b Average tumor growth curves for distant tumors (n = 5 independent samples). c Tumor weights of distant tumors after various treatments (n = 5 independent samples). Box plots show the median (center line), first and third quartiles (edges), and data range (whiskers). d Gross images of distal tumors in each group (n = 5 independent samples). e, f Representative FCM analysis (e) and quantification of matured DCs (f) in primary tumors after different treatments (n = 3 independent samples). g Quantification of CD8⁺ T cells in primary tumors (n = 3 independent samples). h, i Representative FCM analysis (h) and quantification (i) of CD8⁺ T cells in distant tumor (n = 3 independent samples). j Relative quantification of CD8⁺CD69⁺ T cells in distant tumors following different treatments (n = 3 independent samples). k, l Representative FCM plots (k) and relative quantification (l) of macrophage-M1 in distant tumors (n = 3 independent samples). m Quantification of NKT cells in distant tumors (n = 3 independent samples). n, o Representative FCM plots (n) and quantification (o) of MDSCs in distant tumors (n = 3 independent samples). p, Quantification of tumor-infiltrating CD8⁺PD−1⁺ T cells in distant tumors (n = 3 independent samples). q–t Quantification of IFN-γ (q), IL-6 (r), IL-12P70 (s), and TNF-α (t) levels in different groups (n = 3 independent samples). u, v Relative quantification of CD8⁺ T cells (u) and MDSCs (v) in the spleen (n = 3 independent samples). w Multicolor immunostaining images of distant tumors showing CD4⁺, CD8⁺, Foxp3⁺, and DAPI cells in the control and Anti-PD1+wf-UMP groups. Scale bars, 50 μm. Group annotations: G1, Control; G2, US; G3, Anti-PD1; G4, Anti-PD1-PD1+ mKNN MN; G5, Anti-PD1+wf-UMP. All data are presented as mean ± s.e.m. Statistical difference was calculated using a two-tailed unpaired student’s t-test. Significance thresholds: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided as a Source Data file.