Fig. 2: mRNA display peptides bind to the lumen of the BamA β-barrel.
From: Antibacterial macrocyclic peptides reveal a distinct mode of BamA inhibition
Darobactin (A, blue) was previously shown to bind to the lateral gate (strands β1 and β16) of the E. coli BamA β-barrel (PDB 7NRE42). Our x-ray crystallographic analysis shows that, in contrast, CP1 (B, pink), CP2 (C, yellow), and CP3 (D, teal) bind to the lumen of the BamA β-barrel. In (A–D) a periplasmic view is shown on the bottom. E–G The key interactions that mediate the binding of the indicated peptide to the BamA β-barrel are shown.
