Fig. 8: IPA promoted PI3K expression through the ligand binding of AhR in the heart. | Nature Communications

Fig. 8: IPA promoted PI3K expression through the ligand binding of AhR in the heart.

From: Microbiota-indole-3-propionic acid-heart axis mediates the protection of leflunomide against αPD1-induced cardiotoxicity in mice

Fig. 8

A AhR binding site was identified on the PI3K promoter. B Independent ChIP-PCR (n = 6). C, D Relative mRNA levels of Pik3ca in hearts of mice (n = 6). E Representative western blots of p-PI3K, PI3K, p-AKT, and p-GSK-3β protein expression in hearts (n = 6). F Cell viability in αPD1, αPD1+IPA and αPD1 +shPI3K groups (n = 6). Data are shown as the mean ± SEM and analyzed using unpaired two-tailed Student’s t-test (B) or one-way ANOVA with post hoc tests.

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