Fig. 3: Quality control metrics for CUT&Tag data.
From: CUT&Tag recovers up to half of ENCODE ChIP-seq histone acetylation peaks
a Violin and density plot of CUT&Tag fragment size distributions by sample. b Fractions of reads in peaks (FRiPs) defined by ENCODE for H3K27ac and H3K27me3 ChIP-seq, and peaks called for each sample with MACS2 and SEACR. c) Fractions of CUT&Tag sample fragments in ENCODE H3K27ac ChIP-seq and ATAC-seq peaks, as well as their overlapping and unique peaks by excluding (exc.) overlapping regions. d Integrative Genomics Viewer (IGV)29 tracks showing ENCODE ChIP-seq and CUT&Tag (CT) read pileups and their corresponding MACS2 and SEACR peaks in a randomly selected genomic region (chr1:53,289,336-53,822,179). Pileup signals are shown in kilobases (kb).
