Fig. 4: H3K27ac peak calling with SEACR and MACS2.
From: CUT&Tag recovers up to half of ENCODE ChIP-seq histone acetylation peaks
a Precision and recall of MACS2 and SEACR peaks across peak calling parameters (MACS2: q-value; SEACR: proportion of top peaks). b Integrative Genomics Viewer (IGV) tracks for H3K27ac ENCODE ChIP-seq and CUT&Tag (CT) read pileups, alongside MACS2 and SEACR called peak ranges and Refseq genes. Pileup signals are shown in kilobases (kb). c Heatmap of H3K27ac read signal enrichments across a ± 3 kb window centered on peak summits from ENCODE H3K27ac ChIP-seq, and CUT&Tag MACS2 and SEACR, all subsampled to 2 million reads. Rows represent individual H3K27ac peaks and columns show signal values with lighter colors indicating higher signal intensity. d Clustered Pearson’s correlation coefficients between H3K27ac sample peaks called with MACS2 (yellow) and SEACR (orange) created using DiffBind based on binary peak overlap.
