Fig. 3: ALG-2 compensates for the loss of ALG-1 in dpf-3(xe68);alg-1(gk214) animals.

a Representative western blot of ALG-1/2 protein expression in WT, dpf-3(xe68), alg-1(gk214) and dpf-3(xe68);alg-1(gk214) worms. ACTIN acts as a loading control. The numbers on top of the alg-1(gk214) and dpf-3(xe68);alg-1(gk214) bands represent ALG-2 protein signal intensity relative to alg-1(gk214) when normalized on the ACTIN band. N = 4. b Quantification of the signal intensity of the ALG-2 western blot band across four biological replicates, comparing alg-1(gk214) condition to dpf-3(xe68);alg-1(gk214) condition. Data are presented as mean values of four biological replicates (black dots) ± SD. P-value was calculated by two-tailed one sample Student t-test. c let-7 miRISC purification was performed and followed by western blot analysis to measure ALG-1/2 protein association to let-7. The western blot shown is a representative replicate. Input and purifications are separated as they received different exposure time, but were still ran on the same gel. The numbers on top of the alg-1(gk214) and alg-1(gk214); dpf-3(xe68) bands in the let-7 section represent ALG-2 protein signal intensity relative to alg-1(gk214). N = 3. d Quantification of the signal intensity of the ALG-2 western blot band of the let-7 miRISC purifications across three biological replicates, comparing alg-1(gk214) condition to dpf-3(xe68);alg-1(gk214) condition. Data is presented as mean value of three biological replicates (black dots) ± SD. P-value was calculated by two-tailed one sample Student t-test. e alg-2 RNAi was performed on alg-1(gk214) and alg-1(gk214); dpf-3(xe68) L4 animals (5–10 animals per condition per replicate) and the percentage of lethality in the progeny was measured (approx. 100 progeny per condition per replicate). Data are presented as mean values ± SD of three biological replicate. Each dot represents an independent experiment. P-value was calculated by two-way ANOVA. n.s. = non-significant.