Fig. 5: DPF-3 does not co-immunoprecipitate with ALG-2.
From: Dipeptidyl peptidase DPF-3 is a gatekeeper of microRNA Argonaute compensation in animals
a Schematic of endogenously V5 tagged ALG-2 protein by CRISPR-Cas9. The V5 tag is represented in yellow. Amino acids position are displayed at the bottom. b Representative western blot of V5::ALG-2 immunoprecipitation in v5::alg-2 (- lanes) or dpf-3::flag-ha;v5::alg-2 (+ lanes) background. The asterisk highlights a DPF-3 isoform of lower molecular weight than expected. ACTIN acts as a loading control. N = 2. c Representative western blot of V5::ALG-2 immunoprecipitation in dpf-3::flag-ha;v5::alg-2 (- lanes) or dpf-3::flag-ha;v5::alg-2;alg-1(gk214) (+ lanes) background. The asterisk highlights a DPF-3 isoform of lower molecular weight than expected. ACTIN acts as a loading control. N = 2.
