Fig. 6: Absence of DPF-3 in alg-1 loss-of-function animals increases ALG-2 mRNA abundance.
From: Dipeptidyl peptidase DPF-3 is a gatekeeper of microRNA Argonaute compensation in animals
a RT-qPCR performed on total RNA extracts of WT, dpf-3(xe68), alg-1(gk214) and dpf-3(xe68);alg-1(gk214) animals to measure mature ALG-2 mRNA using primers in exon-exon junctions. Data are presented as mean values of 3 biological replicates (white dots) ± SD. ALG-2 mRNA levels were normalized using tba-1. P-values were calculated by two-tailed Student t-test. n.s. = non-significant. b Top: schematic of ALG-2 unspliced mRNA where half-arrows represent roughly the position and orientation of the primers used for qPCR. Bottom: RT-qPCR performed on total RNA extracts of WT, dpf-3(xe68), alg-1(gk214) and dpf-3(xe68);alg-1(gk214) animals to measure ALG-2 precursor mRNA using primers in intronic regions. Data are presented as mean values of 3 biological replicates (white dots) ± SD. ALG-2 pre-mRNA levels were normalized using tba-1. P-values were calculated by two-tailed Student t-test. n.s. = non-significant.
