Fig. 5: Ablation of PfFL, but not VALFL, cells before learning impairs the refinement of initial reach direction.
From: Dissociable roles of distinct thalamic circuits in learning reaches to spatial targets
a Experimental design showing the timeline for surgeries and STT training, and coronal ABA sections showing the injection targets for PfFL and VALFL bilateral lesions. b Representative histology of Pfpre-lesion mouse, dashed-line indicating Pf. NeuroTrace (yellow), with high-magnification view from the inset in box. c Same as (b) for Pfpre-control mouse. d, e Same as (b, c), for VALpre-lesion and VALpre-control mice. Dashed-line indicating VAL. f Hit ratio on five selected days over two blocks; Early (E) day of block, Late (L) day of block, and three equidistant days in between. Pfpre-lesion (purple) and Pfpre-control (gray). 2-way repeated measures ANOVA: Block 1, group: F(1,18) = 16, *p = 0.0016, Block 2: group: F(1,18) = 6, *p = 0.0071; Šídák’s multiple comparison test Pfpre-lesion vs Pfpre-control late block 1,*p < 0.0001; late block 2, *p = 0.0028. g Same as E, but for VALpre-lesion (green) and VALpre-control (gray). 2-way repeated measures ANOVA: Block 1, group: F(1,16) = 1.9, p > 0.05, Block 2: group: F(1,16) = 0.07, p > 0.05. h Variability of initial direction for all trajectories. 2-way repeated measures ANOVA; Block 1, group: F(1,18) = 12, *p = 0.0078, Block 2: group: F(1,18) = 5, *p = 0.0385; Šídák’s multiple comparison test Pfpre-lesion vs Pfpre-control late block 1,*p = 0.0348. i Same as in (h) for VAL lesion groups. 2-way repeated measures ANOVA: Block 1, group: F(1,16) = 0.002, p > 0.05, Block 2: group: F(1,16) = 0.84, p > 0.05. j Peak speed of all trajectories. 2-way repeated measures ANOVA: Block 1, group: F(1,18) = 0.16, p > 0.05, Block 2: group: F(1,18) = 2.2, p > 0.05. k Same as in (j), for VAL lesion groups. 2-way repeated measures ANOVA: Block 1, group: F(1,16) = 4, *p = 0.02, Block 2, group: F(1,16) = 5, *p = 0.0392. l Overshoot of target for hit trajectories for Pf lesion groups. 2-way repeated measures ANOVA: Block 1, group: F(1,16) = 2.5, p > 0.05. m Same as in (l), for VAL lesion groups. 2-way repeated measures ANOVA: Block 1, group: F(1,16) = 7, *p = 0.0124. f–m Asterisks show ANOVA effect of group (lesion vs control) in block. Mean ± SEM with thick colored lines and shaded bounds. Pfpre-lesion, n = 8 mice; Pfpre-control, n = 10 mice; VALpre-lesion, n = 7 mice; VALpre-control, n = 10 mice. See also Supplementary Fig. 10. Source data are provided as a Source Data file.
