Fig. 5: Epitopes analysis of B026 and B019.

A Modeled structure of the B6R protein predicted using AlphaFold2. The four domains of B6R are shown in red (domain 1), blue (domain 2), yellow (domain 3), and green (domain 4). The stalk region is shown in brown. B Schematic diagrams representing the structures of a series of truncated B6R proteins variants. All these truncated B6R constructs contain a 6×His tag at the C-terminus, which was used for protein purification. C, D Binding of B026 (C) and B019 (D) to different fragments of B6R. Different fragments of B6R were loaded onto the surface of biosensors, and individual antibodies were tested at three concentrations (75 nM, 150 nM, 300 nM). The association and dissociation curves are shown. The dashed lines represent the fitted curves based on the experimental data. Source data are provided as a Source Data file.