Fig. 4: In vitro and in vivo biocompatibility of oHA microparticles. | Nature Communications

Fig. 4: In vitro and in vivo biocompatibility of oHA microparticles.

From: Covalently reactive microparticles imbibe blood to form fortified clots for rapid hemostasis and prevention of rebleeding

Fig. 4

a In vitro cell viability of mouse fibroblast (L929) cells assessed using a LIVE/DEAD assay after 24 h of co-incubation with or without 20 mg of xHA or oHA microparticles (n = 5 independent samples). b Hemolysis rates of xHA and oHA microparticles, along with representative digital images (n = 5 independent samples). c Timeline of the subcutaneous implantation experiment for evaluating in vivo biocompatibility and biodegradation. Created in BioRender. Chen, T. (2025) https://BioRender.com/x73e121. d Representative histology images with H&E staining on days 1 and 7 post-subcutaneous implantation. Black arrows indicate the infiltration of inflammatory cells. e Representative digital photographs and histology images with H&E staining of the subcutaneous tissue from the oHA and xHA microparticles-implanted groups at 1 day, 1 week, 1 month, and 3 months post-implantation. Black triangles indicate residual microparticles. Data are presented as means ± s.d. Source data are provided as a Source Data file.

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