Fig. 4: Light-controlled expression of a fluorescent protein in zebrafish embryos. | Nature Communications

Fig. 4: Light-controlled expression of a fluorescent protein in zebrafish embryos.

From: Optochemical control over mRNA translation by photocaged phosphorodiamidate morpholino oligonucleotides in vivo

Fig. 4

Zebrafish embryos were injected with the mRNA encoding for the nuclear GFP (blue), cPMO2 (yellow with black stripes and light-cleavable moieties in pink), as well as with the standard translation-blocking morpholino (black). Embryos were allowed to develop until 4 hours post fertilization (hpf). a Some of the embryos were kept in the dark (upper arrow), such that the translation of the mRNA was blocked by the translation-blocking morpholino. In this case, no expression of nuclear GFP protein was detected (upper right panel). b Sibling embryos were subjected to local irradiation with the 405 nm laser (lower panels, red dotted box), resulting in the photolysis of PMO2’s light-sensitive moieties. This procedure resulted in sequestration of the translation-blocking morpholino, thus allowing the translation of the nuclear GFP, which was visualized 2 h post-irradiation (green spots). Scale bar 20 μm. Source data are provided as a Source Data file.

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