Table 1 Thermal melting temperature of duplexes. Structures and linkages of the oligos are shown in Fig. 1

From: Optochemical control over mRNA translation by photocaged phosphorodiamidate morpholino oligonucleotides in vivo

Oligo

Sequence

With complementary tbMO Tm (oC)

RNA

5′-uauaaauuguaaaugagguaagagg-3′ (target RNA sequence)

48

PMO1

5′-TATAAATTGTAACTGAGGTAAGAGG-3′

36

PMO2

5′-T^A^T^A^AATTGTAACTGAGGTAAGAGG-3′

34

PMO3

5′-T^A^T^A^AATTGTAACTGAGGTAAGAGG-3′

37

cPMO2

5′-T^A^T^A^AATTGTAACTGAGGTAAGAGG-3′(before UV exposure)

24

PMO2

5′- T^A^T^A^AATTGTAACTGAGGTAAGAGG-3′ (after UV exposure)

35

  1. Measurements were performed in 40 mM phosphate buffer (pH 7) with a concentration of 2 μM PMO (each strand) with complementary tbMO sequence (5’-CCTCTTACCTCAGTTACAATTTATA-3’). Tm values reported are the averages of two independent experiments that were within ±1.0 °C. Tm values were calculated from the first derivative plot.
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