Fig. 4: ATF3-binding is enriched in genes with NS1-Mediator peaks and differential expression in hRSV infection.
A Schematic representation of the integrative analysis that defined overlapping NS1-Mediator ChIPseq peaks from A549 and hTBEC cells transfected with NS1 or empty vector, or infected with hRSV with WT or Y125A mutant NS1; overlapping TF ChIPseq peaks from the ReMap2022 database; and genes bound by these factors that are differentially expressed in cells treated the same as for the NS1-MED ChIPseq. B Functional genome annotations of NS1 and NS1-Mediator peaks show an enrichment of proximal promoter binding in the latter compared to the former. C Venn diagram representation of the integrative analysis results. D Heatmap shows Z-score expression of DEGs in RSV-infected vs mock treated hTBECs [padj < 0.05, log2 FC > abs(1)]. E Barplot shows enriched gene pathways in DEGs from (D). F Lollipop plot shows the number of DEG targets and log2 FC expression of TFs with the highest number of overlapping NS1-Med peaks and DEG targets. G Schematic shows the overlap of NS1 and Mediator CUT&RUN peaks and the proportion of those with ATF3 CUT&RUN peak overlaps. H Heatmap plotted as in D shows DEGs with NS1-Mediator-ATF3 overlapping CUT&RUN peaks. I Volcano plot of DEGs in H with selected ATF3-bound genes labeled in blue, remainder of DEGs in salmon, and non-DEGs in gray. D–F, H–I: padj – FDR adjusted p value.
