Fig. 4: Expression of an efficient deoxynucleoside kinase from Drosophila melanogaster promotes telomerase activity from dG treatment and lengthens telomeres.
From: Metabolic constraint of human telomere length by nucleotide salvage efficiency
a Schematic of Dm-dNK activity. b Immunoblot for V5-tagged Dm-dNK in TERC −/− 293 T cells stably expressing Dm-dNK. The results represent a single experiment. c–f TRF Southern blot of TRACE assay from TERC −/− 293 T cells overexpressing either eGFP or Dm-dNK which were transfected with the indicated doses of dG, guanine, or guanosine for 30 h. Experiments performed in biological duplicate (c–e) or biological triplicate (f). g TRF Southern blot of 293 T cells which were transduced with lentivirus for constitutive overexpression of eGFP or Dm-dNK and cultured for one month. A representative blot is shown from three biological replicates. h TRF Southern blot of K562 cells which were transduced with lentivirus for doxycycline inducible overexpression of eGFP or Dm-dNK and cultured for one month in the presence of 1 μg/mL of doxycycline. A representative blot is shown from two biological replicates. i Quantification of g and h. Data are presented as means and error bars indicate s.d. P-values were calculated using a paired two-sided t test. Source data are provided as a Source Data file.
