Fig. 5: Manipulation of deoxynucleotide salvage by expressing Dm-dNK or treatment with dG plus ulodesine drives telomere lengthening in fibroblasts from patients with TBDs.
From: Metabolic constraint of human telomere length by nucleotide salvage efficiency
a TRF Southern blot from TERT expressing fibroblasts from a healthy donor overexpressing eGFP or Dm-dNK as indicated and cultured for five weeks. b TRF Southern blot from TERT expressing fibroblasts from TBD patients harboring mutations in the indicated genes overexpressing eGFP or Dm-dNK as indicated and cultured for one month. c Quantification of median telomere length from b. Error bars represent s.d. P value was calculated using a paired two-sided t-test. d TRF Southern blot from TERT transformed fibroblasts from a healthy donor which were treated with 1 µM ulodesine and dG as indicated for three weeks. e TRF Southern blot from TERT transformed fibroblasts from TBD patients harboring mutations in the indicated genes which were treated with 1 µM ulodesine and dG as indicated for three weeks. f Quantification of median telomere length from e. Statistical significance determined using paired two-sided t-test. Error bars indicate standard deviation. The results from a and d represent single experiments. Source data are provided as a Source Data file.
