Table 1 . Cryo-EM map and model statistics, Related to Figs. 1, 3 and 5
Data collection and processing | BLOC3 complex | HPS1-HPS4-Rab9A Q66L complex |
|---|---|---|
Magnification | 165,000 | |
Voltage (kV) | 300 | |
Pixel size (Å) | 0.85 | |
Symmetry imposed | C1 | |
Micrographs acquired | 10,755 | 11,110 |
Electron exposure (e-/Å2) | 52.1 | 61.6 |
Defocus range (μm) | − 3.0 ~ − 0.1 | − 5.5 ~ − 0.1 |
Initial particle images (no.) | 1,118,353 | 851,793 |
Final particle images (no.) | 213,712 | 93,089 |
Map resolution (Å) | 3.19 | 4.4 |
FSC threshold | 0.143 | 0.143 |
Map resolution range (Å) | 3.1 ~ 5.1 | 3.5 ~ 6.4 |
Model refinement | ||
Model resolution (Å) | 3.5 | N/A |
FSC threshold | 0.5 | N/A |
Map sharpening B factor (Å2) | − 128 | N/A |
Model composition | ||
Non-hydrogen atoms | 7608 | N/A |
Protein residues | 949 | N/A |
B factor (Å2) | − 79.88 | N/A |
R.m.s. deviations | ||
Bond lengths (Å) | 0.002 | N/A |
Bond angles (°) | 0.516 | N/A |
MolProbity score | 1.38 | N/A |
Clashscore | 5.30 | N/A |
Ramachandran plot | ||
Favored (%) | 9751 | N/A |
Allowed (%) | 2.49 | N/A |
Outliers(%) | 0.00 | N/A |
