Fig. 4: W109^2.60 exerts a distinct effect in regulating signaling downstream to both VUFs.

a Stimulation of CXCR3^W109A (red) with VUF10661 fails to elicit any response in G-protein signaling and βarr1 recruitment assays, while VUF11418 induces a slightly enhanced response, as compared to CXCR3^WT (black). Data (mean ± SEM) represents three (for G-protein assays) or five (for βarr1 recruitment) independent experiments, performed in duplicate, and has been normalized with respect to the signal observed at the lowest ligand concentration, treated as either 100% (for G-protein assays) or 1 (for βarr1 recruitment). b W109^2.60 in CXCR3 interacts with VUF10661, while rotation of this residue in the VUF11418-bound structure prevents its interaction with VUF11418. (VUF10661-CXCR3: olive drab, VUF10661: cyan, VUF11418-CXCR3: pale violet red, VUF11418: deep teal). Source data are provided as a Source Data file.