Fig. 1: TaSGR1 positively regulates wheat resistance to Pst.

a TaSGR1OE and Fielder plants were inoculated with Pst CYR31 and CYR34, and disease phenotypes were observed at 14 dpi. b The Pst/wheat biomass ratio in infected leaves at 120 hpi was measured using qPCR. c Chl contents decreased in Pst CYR31-infected TaSGR1OE plants at 5 dpi. d H₂O₂ accumulation was observed after DAB staining in the leaves of Pst CYR31-infected TaSGR1OE wheat plants at 24 or 48 hpi. Bar = 20 mm. SV substomatal vesicle, IH infectious hyphae. e Quantification of infection areas containing H₂O₂ in Fielder and TaSGR1OE plants infected with Pst CYR31 at 24 and 48 hpi. Means ± standard deviation (SD) were calculated from 30 infection sites across three independent biological replicates from three leaf samples of different plants. The P value was determined using a two-tailed unpaired Student’s t test. f Fielder and tasgr1-ABD plants were inoculated with Pst CYR23, and disease phenotypes were observed at 14 dpi. g The Pst biomass in infected leaves was measured using qPCR. h Relative expression of the marked defense-related genes in tasgr1-ABD plants at 0 and 24 hpi with Pst CYR23. Transcript levels were confirmed via qPCR and normalized to TaEF1a. i Chl contents in Pst CYR23-infected tasgr1-ABD plants decreased at 5 dpi. In (b, c, g, h, and i), mean values and SD were determined (n = 3 biologically independent samples). The P value was determined using a two-tailed unpaired Student’s t test. Source data are provided as a Source Data file.