Fig. 2: TaSGR1 interacts with the positive resistance regulator TaTrx.
From: A fungal pathogen suppresses host leaf senescence to increase infection
a Detection of the TaSGR1–TaTrx interaction via Y2H assays. P53/SV40-T and TaSGR1/AD were used as the positive and negative controls, respectively. b Interaction of TaSGR1 with TaTrx detected in the chloroplasts of N. benthamiana leaves transiently expressing the marked constructs via BiFC assays. TaSGR11–51, the cTP of TaSGR1; TaTrx1–59. CTP1 fused with a CFP was used as a marker protein to localize into chloroplasts. Bar = 20 μm. c Confirmation of the TaSGR1–TaTrx interaction via Co-IP assays. Western blots of total proteins extracted from N. benthamiana leaves transiently expressing the marked constructs and proteins eluted from GFP-trap beads were detected using anti-GFP and anti-HA antibodies. d The STAYGREEN domain of TaSGR1 interacted with TaTrx in yeast cells. TaSGR155-207 contained the STAYGREEN domain. e Fielder and tatrx plants were inoculated with Pst CYR23, and disease phenotypes were observed at 14 dpi. f The Pst/wheat biomass in infected leaves was measured using qPCR. Means ± SD were determined based on three biological replicates from three leaf samples of different plants. The P value was determined using two-tailed unpaired Student’s t test. Source data are provided as a Source Data file.
