Fig. 4: Monomeric TaSGR1 promotes plant resistance to Pst.
From: A fungal pathogen suppresses host leaf senescence to increase infection
a Monomeric TaSGR1 contributed to Pst resistance in wheat. TaSGR15A and TaSGR1 were transiently expressed in tatrx plants, respectively. Leaves were inoculated with Pst CYR31, and disease phenotypes were observed at 14 dpi. b The Pst/wheat biomass ratio reported in (a) was assayed using DNA isolated from the second leaves at 120 hpi. TaEF1α and PstEF were used to normalize the DNA levels of wheat leaves and Pst, respectively. c Semi-in vivo activity of TaSGR1 and its mutants. Spectrophotometric measurements of the Chl contents in crude Chl extract mixtures of N. benthamiana with the TaSGR1 protein were conducted as described in the methods. d TaTrx promoted Chl degradation by TaSGR1 in the semi-in vivo assay. Mixtures of equal amounts of TaTrx or TaTrxM and TaSGR1 were incubated with crude Chl extract, and Chl consumption was measured using a spectrophotometer. In (b–d), means ± SD were determined based on three biological replicates from three leaf samples of different plants. The P value was determined by two-tailed unpaired Student’s t test. Source data are provided as a Source Data file.
