Fig. 1: Transfer of activated and tumor antigen-loaded splenic cDC1s outperforms cDC2s in inducing a cancer-specific CD4+ Th1 and CD8+ T cell effector response.
a Experimental overview for b-c: splenic cDC1s and cDC2s were incubated with B16-OVA TCL and TLR agonists for 1, 4, and 16 h (overnight, ON) and co-cultured with cell trace violet (CTV)-labeled naive OT-I or OT-II cells. b Flow cytometric analysis of frequency of divided OT-I and OT-II cells (n = 3 biological replicates/group from 3 independent experiments). c Representative histograms depicting CTV dilution for OT-I and OT-II co-cultured with 1 h (cDC1s) or 4 h (cDC2s) TCL+CpG-pulsed cDCs. d Experimental overview for (e, f): B16-OVA TCL + TLR agonist-treated cDC1s (1 h) and cDC2s (4 h) were injected intradermally in the flank of mice. Controls received PBS injection. IFNγ production by T cells in the iLN upon restimulation was assessed 7 days later. e Log-transformed number of activated CD44+ IFNγ+ CD8+ or CD4+ T cells in iLN (left to right: n = 10, 10, 10, 11, 7, 8, 7 [OVA257-264]; 16, 15, 19, 17, 12, 14, 13 [OVA323-339] biological replicates/group from 4 independent experiments). f Representative dot plots of CD8+ and CD4+ T cells from the iLN of mice untreated or treated with TCL+CpG-incubated cDC1s or cDC2s. g Experimental overview for (h, i): MC38 TCL+CpG-pulsed cDC1s (1 h) and cDC2s (4 h) were injected intradermally in the flank of mice. Controls received PBS injection. 7 days later, the IFNγ, TNFα, and IL17A production by CD4+ T cells in the iLN upon restimulation with MC38 TCL-loaded APCs was evaluated. h, Frequency of CD44+ IFNγ+, TNFα+, and IL17A+ in CD4+ T cells in iLN (n = 15 [PBS], 20 [cDC1], 16 [cDC2] biological replicates/group from 4 independent experiments). i Representative dot plots of CD4+ T cells of mice untreated or treated with CpG+TCL-incubated cDC1s or cDC2s. Data are presented as mean ± SEM (b) or box plots where whiskers represent minimum and maximum values, boxes indicate median, 25th, and 75th percentiles (e, h). Dots represent individual data points. Statistical analysis by paired (b) or unpaired (e, h) one-way ANOVA Tukey post-hoc test. Source data are provided as Source Data file.
