Fig. 5: cDC1 anti-cancer vaccination blocks experimental cancer recurrence after tumor resection. | Nature Communications

Fig. 5: cDC1 anti-cancer vaccination blocks experimental cancer recurrence after tumor resection.

From: Immunotherapy with conventional type-1 dendritic cells induces immune memory and limits tumor relapse

Fig. 5

a, b Experimental overview for (c–f): 5×105 MC38 cells were intradermally (i.d.) injected into naive mice (flank), and cancer growth monitored until day 10, when tumors were resected. Mice received adjuvant (post-surgery, day 13 and 18) (a, c, d) or neoadjuvant (pre-surgery, day 3 and 8) (b, e, f) treatments with anti-PD1 antibody intraperitoneally (i.p.), 106 MC38-TCL loaded cDC1s (i.d.), or PBS control. 30 days after the last intervention, 1.5 × 106 MC38 cells were reinjected (same flank) and tumor growth (c, e) and survival (d, f) monitored. Tumor naive mice only received MC38 cells at day 48 (c, d; n = 11 [control], 11, [anti-PD1], 12 [cDC1], 8 [naïve]) or 40 (e, f; n = 10 [control], 12, [anti-PD1], 9 [cDC1], 7 [naïve]). n represents biological replicates/group from 2 independent experiments). g Experimental overview for (h, i): 105 B16-F10 cells were i.d. injected and on day 12 tumors were resected. On days 4 and 10, mice were treated with B16-F10-TCL loaded cDC1s (106, i.d.), or control PBS. On day 39, 7 × 105 B16-F10 cells were i.d. injected in the same flank, and tumor growth (h) and survival (i) monitored. Tumor naive mice were only injected with 7 × 105 B16-F10 cell at day 39 (n = 6 [control], 7 [cDC1], 7 [cDC2] biological replicates/group from one experiment). j Experimental overview for (k, l): 5 × 105 MC38 cells were i.d. injected and on day 10 tumors were resected. On days 3 and 8, mice were treated with MC38-TCL loaded cDC1s (106, i.d.), or control PBS. At day 49 and 51 after the initial tumor graft, cDC1-treated mice were injected with anti-CD4, anti-CD8b depleting antibodies or rat IgG as control i.p. On day 52, 1.5 × 106 MC38 cells were i.d. injected in the same flank of mice, and tumor growth (k) and survival (l) monitored (n = 9 [control], 9 [cDC1+IgG], 10 [cDC1+anti-CD8], 9 [cDC1+anti-CD4] biological replicates/group from 2 independent experiments). Data are presented as mean ± SEM. Statistical analysis by two-way ANOVA (c, e, h, k) and Mantel-Cox test (d, f, i, l). Source data are provided as Source Data file.

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