Fig. 6: Landscape of alternative splicing event types.

A Number of events by types. Bar length indicates the number of canonical events of each type predicted from genome annotation. Dark gray: events that did not display DAS between neuron types in our dataset; dark red: events DAS in at least one pair of neurons; dark blue: events DAS between tissues, based on published tissue-specific TRAP-Seq data, but not between individual neuron types in our data; light gray: events that could not be measured in either dataset. B Distribution (density plot) of length or conservation score of particular sequences within splicing events, for DAS events (red) vs non-DAS events (gray). Only features with a statistically significant difference between DAS and non-DAS events are represented here. Vertical dashed lines represent the median of each group. Lengths in basepairs (bp). C Histogram of cassette exon lengths, separating DAS exons (red) and non-DAS exons (gray). The vertical dashed bar at 27 bp delimits microexons. D For each exon (each dot), proportion of neuron pairs where the exon is differentially AS between the two neuron types (proportion calculated among the neuron pairs in which the exon-containing gene is expressed in both neurons of the pair). p = 0.0035 (two-sided Wilcoxon test). E Potential impact of nucleotides addition on open reading frame. For alternative splice sites and cassette exons overlapping a CDS, the color indicates whether inclusion of the alternative event results in a nucleotide addition that is a multiple of 3 (lilac) or is not a multiple of 3 (green), separating events DAS between neuron types. Alt. 3’ ss, p = 0.023; alt. 5’ ss, p = 0.053; cassette exon, p = 0.41 (Chi-square test, comparison of the proportion of PFS events between DAS and non-DAS events). F Location of events within the gene body. For alternative splice sites and cassette exons, the color indicates non-PFS (lilac) and PFS events (green). The density curve indicates the distance from the event to the closest extremity (start or end) of the gene. ***: p < 0.001 (two-sided Wilcoxon test, comparison of distance from extremity between PFS and non-PFS events, test statistics reported in Source Data). Source data are provided as a Source Data file.