Fig. 2: In vitro characteristics of λS-LC species. | Nature Communications

Fig. 2: In vitro characteristics of λS-LC species.

From: A mouse model of cardiac immunoglobulin light chain amyloidosis reveals insights into tissue accumulation and toxicity of amyloid fibrils

Fig. 2

A Stability of the recombinant λS proteins, rλS-LC (pink) and rλS-VL (green) was measured by the ratio of the fluorescence at 350 and 330 nm (solid line) upon thermal denaturation. The melting temperature (Tm) for each sample was given by the maximum peaks of the 350/330 nm ratio first derivative (dotted line). The curves correspond to the mean of 3 different measurements for the rλS-LC and 4 for the rλS-VL, and the error bars correspond to SD. B Kinetics of fibril formation in vitro with the different λS protein species with (light green and pink) or without seeding (dark green and pink). The aggregation kinetics was followed by the measurement of the fluorescence of Thioflavin T. Represented in mean ± SD of 3 different experiments in duplicate. C Representative TEM analysis of the samples from (B) without (upper panel) or with (lower panel) seeding.

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