Fig. 6: Comparison of L–VP35 interactions between MARV and EBOV polymerases. | Nature Communications

Fig. 6: Comparison of L–VP35 interactions between MARV and EBOV polymerases.

From: Structural insights into the RNA-dependent RNA polymerase complexes from highly pathogenic Marburg and Ebola viruses

Fig. 6

a Superimposition of eVP35 (PDB: 7YER) onto mVP35 (this work) based on the alignment of OD and CR. MARL and mVP35 are shown and colored the same as in Fig. 5a. eVP35 is shown as ribbon and colored in white. A 6.5° rotation between eVP35 and mVP35 IIDs and the distances of α4 and α6 movements are indicated. b Superimposition of eVP35 (PDB: 7YER) onto mVP35 (this work) based on the alignment of IID. A 7–8 Å shift and 4.5° rotation between eVP35 and mVP35 ODs are indicated. c–e Zoom-in views of the blue boxes in a and b. MARL and mVP35 tetramer are depicted as ribbon and colored as in Fig. 5. EBOL and eVP35 are shown in light gray ribbons. Residues that involved in the interactions between MARL and mVP35 are shown as sticks. Residues that involved in the interactions between EBOL and eVP35 are shown in lines. Polar interactions between MARL and mVP35 are represented by black dashed lines, and those between EBOL and eVP35 are displayed by red dashed lines. Putative polar interactions between MARL and eVP35 are represented by gray dashed lines, and those between EBOL and mVP35 are represented by green dashed lines. The yellow pentacle indicates the predicted clash between Tyr176 of eVP35 and Tyr706 of MARL.

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