Fig. 2: Cryo-EM density map (A) and model (B) of the GCase/sLIMP-2/PMbs tetramer.
From: Cryo-TEM structure of β-glucocerebrosidase in complex with its transporter LIMP-2
A B-factor-sharpened map of electron density obtained from cryo-EM dataset (consensus refinement, resolution: 3.68 Å; B-factor: -152.7 Å2; level: 0.08; dust hidden < 11 Å). Surface colored according to the obtained model (see B). B Structure of sGCase/LIMP-2/PMb based on the obtained map shown in cartoon style with glycans shown as ball and stick. Blue: sLIMP-2; orange: GCase; green: GCase active site; light purple: Nb1; dark purple: Nb6; white: glycans. The orientation of LIMP-2 at the membrane is illustrated and the N- and C- terminal transmembrane helices present in the full-length protein are indicated (map and model are deposited in PDB as 9FJF and EMDB as EMD-50502).
