Fig. 5: VL-induced macrophage heterogeneity revealed by single-cell RNA sequencing.

a UMAP plot of scRNA-seq data from sorted live, single, CD45.2⁺F4/80⁺CD11b^intCD64⁺ cells from livers of uninfected and 42 d.p.i. mice, showing three main clusters for naïve and seven clusters for infected mice. b Annotated UMAP plot showing the single-cell expression of conserved KC signature genes as described by Guilliams et al.¹⁹ c Single-cell expression of Ccr2, Clec4f, and Timd4 in CD45.2⁺F4/80⁺CD11b^intCD64⁺ cells from naïve and 42 d.p.i. mice. d Average gene expression and the percentage of cells expressing each gene within the identified clusters in naïve and 42-day infected CD45.2⁺F4/80⁺CD11b^intCD64⁺ cells. e Frequency of the different subsets identified by scRNA-seq in naïve and 42 d.p.i. mice. f Heatmap showing the differential expression of Mac2-specific genes identified by Remmerie et al.¹⁴. Data include 1200 naïve cells and 6152 cells from 42 d.p.i. mice after QC filtering.