Fig. 1: MEOX2 and SRGN are enriched in CL and MES GSCs, respectively. | Nature Communications

Fig. 1: MEOX2 and SRGN are enriched in CL and MES GSCs, respectively.

From: Combined targeting of glioblastoma stem cells of different cellular states disrupts malignant progression

Fig. 1

a Composition of four cellular states GSC-like tumor cells (SOX2+ in conjunction with CD133+/CD15+/OCT4+) in each GBM sample from scRNA-seq datasets. b Kaplan–Meier survival curves are shown for GBM patients in our in-housed generated scRNA-seq dataset, based on combination of GSC-like tumor cells proportion as indicated (high: >50%, low: <50%). c Diagram depicting the screening workflow to identify targets for CL/AC-like and MES/MES-like subtype GSCs. Created in BioRender. Wang, X. (2025) https://BioRender.com/f42j224. d Kaplan–Meier survival curve of GBM patients from TCGA (HG-U133A) dataset, based on MEOX2 and SRGN expression (high: >50%, low: <50%). e Bubble plot showing proportions and normalized expression levels of MEOX2 and SRGN in different cellular state GSC-like tumor cells from scRNA-seq datasets. f MEOX2 (left) and SRGN (right) gene expression in the GSCs (n = 3)/DGCs (n = 3) RNA-seq dataset (in-house generated). *p < 0.05. g MEOX2 and SRGN protein levels were measured by western blotting in CL/MES DGCs (n = 3) and CL/MES GSCs (n = 3). Tubulin was used as the loading control. h MEOX2 and SRGN protein levels were measured by western blotting in NSCs (n = 2) and GSCs (n = 10). Tubulin was used as the loading control. Immunofluorescence images showing co-staining of DAPI (blue), SOX2 (green), CD45 (red) with MEOX2 (yellow, i) or SRGN (purple, j) in glioblastoma samples. Scale bar, 2 mm (left), 100 μm (middle and right). The correlations between MEOX2/SRGN, SOX2, and CD45 expressions are shown. The gray area indicates the 95% CI of the regression. k Pan-cancer analysis of MEOX2 (left) and SRGN (right) expression levels in 24 different tumors from the TCGA dataset. **p < 0.01. Box plots show median (center line), 25th–75th percentiles (box bounds), and whiskers extend to minimum and maximum values within 1.5 times the interquartile range. Statistics: (b, d) Log-rank test. e, f, k Unpaired Student’s t test for two-group comparison. i, j The Pearson correlation test. Images: g, h Representative blots (n = 3). i, j Representative images (n = 30). Source data are provided as a Source Data file.

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