Fig. 3: Concurrent IFNα and IL-12 expression ameliorates the fitness of CD4⁺ and TA-specific CD8⁺ T cells.

A UMAP projection of scRNA-seq of liver T and NK cells subclustered. B Gene set enrichment analysis (GSEA) of scRNA-seq data showing normalized enriched score (NES) for selected gene ontology (GO) terms calculated based on genes differentially expressed in T and NK cells in the indicated comparisons (n = 2, 3, 3, 3 mice/group for IiOVA, OVA.Ifna, OVA.Il12 and OVA.Combo). C Combined gene expression score for genes belonging to the indicated categories in liver CD8⁺ T cells. D Fraction of positive cells and scaled averaged expression of selected genes belonging to the indicated biological processes in the liver CD8⁺ T cells (number of mice as in (B). E UMAP projection of liver scRNA-seq indicating cells bearing OVA-specific TCRs. F GSEA of scRNA-seq data showing NES for selected GO terms calculated based on genes differentially expressed in OVA-specific CD8⁺ T cells in the indicated comparisons (number of mice as in B). G Combined gene expression score of genes belonging to the indicated categories in OVA-specific CD8⁺ T cells. H Clonotype sharing between liver and tumor CD4⁺ T cells, grouped by TCR clonotype. I UMAP projection of liver scRNA sequencing indicating CD4⁺ T cells clonotypes shared between liver and tumor tissue. J Percentage of cells within the CD4⁺ T cells populations in the liver (n = 2, 3, 3, 3 mice/group for IiOVA, OVA.Ifna, OVA.Il12, and OVA.Combo, horizontal line represents mean, error bars represent the SEM). K Number of CD4⁺ T cells divided by TCR clonotype frequency.