Fig. 2: Cellular internalization of monomeric tau is inhibited by anti-tau VHHs A31 and H3-2.
From: Inhibition of tau neuronal internalization using anti-tau single domain antibodies
a Inhibition of cellular uptake of monomeric tau1N4R by the anti-tau VHHs. Percentage of cellular tau uptake based on Alexa546 fluorescence signal coming from monomeric tau1N4R (50 nM) co-incubated with PBS (tau_M), RAP (100 nM), heparin (50 µg/mL), and the VHHs (100 nM). n = 4 independent experiments per condition. Data were normalized to the condition of tau1N4R pre-incubated with PBS as 100% of tau uptake. Box plots show the median (center line), 25th, 75th percentiles (box), and minimum and maximum values (whiskers), all data points are shown (single points). Data were analyzed using one-way nonparametric ANOVA (Kruskal-Wallis) with Dunn’s multiple comparison test ****p < 0.0001; ***p < 0.001). RAP, VHHs A31 (p = 0.0004), and H3-2 significantly reduced cellular tau uptake. b Confocal microscopy analysis of cellular tau uptake assay using recombinant tau1N4R labeled with Alexa546 dye and in the presence of PBS (tau_M), RAP, heparin, and VHHs A31, Z70mut1 or H3-2 (images are representative of a n = 2 independent experiments per condition). Tau was visualized in red, and nuclei in light blue. The scale bar is 10 µm.
